Overall a good talk. There were a couple of things that I thought could be improved on:

1. I think if you focused more on what TurboGears currently supports that would be good. It seemed like several times in the talk you bounced around on version numbers (e.g. "in TG 1.x we did this. Now we're at 2.x and we do this, but in the latest 1.y we will be doing this"). IMHO it would be better to say "this is what we're using, but we allow you to plugin x, y, or z if that's what you want", and you don't really need to detail x, y, or z because if people want to use them they'll figure them out because they have specific reasons.
2. At one point you backed up about 10-15 slides just to point out that LAMP doesn't necessarily cover all needs and that some built in functionality to TG is just fine for starting out (IIRC this was related to the Static File Server). I don't think you really needed to show the slide to make your point in that case, and having to run back and forth through the slides breaks the flow of your talk.
3. I think you were showing some nerves during the talk with a little fidgeting (both physical and verbal), which is a little distracting. I think that you had a good talk, so just keep on giving it (and more talks) and just work on relaxing a bit more. Easier said than done, I know, but it's the only way to get better.